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EVOLUTION OF BARBELLUS Objectives: Demonstrate one technique for hypothesizing about the evolutionary relationships among fossil organisms. Demonstrate the use of the “Law of Superposition.” Construct a phylogenetic tree based on fossil evidence. Background: To observe changes in organisms that only take place over long periods of time, scientists often use fossils. Phylogenetic trees, or family trees, may then be constructed to illustrate the relationships of these organisms. Besides the physical appearance of the fossil, the scientist may also be able to draw upon other information. Some fossils, for example, may be laid down in rock layers, or strata, above or below other strata. This provides a clue as to the relative ages of the organisms being studied. In this investigation, assume that all of the fossils of the imaginary genus, Barbellus , came from rock layers exposed along a stream bank, as shown in Figure 3. Sometimes, the fossil record may seem to be incomplete.